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Published on: March 12, 2015
IGM and IGG response to 29-35-kDa Toxoplasma gondii protein fractions in experimentally infected goats
M M Conde de Felipe1, J M Molina, E Rodríguez-Ponce
1Parasitic Diseases Unit, Faculty of Veterinary Medicine, University of Las Palmas de G. C., Trasmontaria s/n, 35416-Arucas, Las Palmas, Spain.
Abstract:
The evolution of the humoral responses of IgG and IgM against 29-35-kDa Toxoplasma gondii fractions from experimentally infected goats were studied and compared by ELISA with the use of whole T. gondii soluble extracts and 29-35-kDa electroeluted proteins as antigens. The IgM response to electroeluted proteins was detected from wk 1 to wk 3 postinfection, showing a similar evolution to that observed when T. gondii crude extracts were used as antigens. These results suggest that this group of proteins could be used for a more specific detection of anti-T. gondii IgM. In the same way, the IgG response was equivalent in both cases, although when 29-35-kDa T. gondii fractions were used as antigens, the level of specific IgGs reached a peak 2 wk before than when T. gondii crude extract was used. The detection by ELISA of anti-T. gondii IgM in goats does not seem to be affected by the presence of specific IgG in serum samples when 29-35-kDa protein fractions were used as antigens.
