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Urinary Bladder

Urinary Bladder

The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...

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The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation

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Bladder afferent sensitivity in wild-type and TRPV1 knockout mice.

D Daly¹, W Rong, R Chess-Williams

¹University of Sheffield, Department of Biomedical Science, Western Bank, Sheffield S10 2TN, UK.

The Journal of Physiology

|July 14, 2007

Summary

The transient receptor potential vanilloid 1 (TRPV1) channel plays a key role in bladder afferent sensitivity, particularly in low-threshold fibers. This finding suggests TRPV1 as a potential therapeutic target for lower urinary tract disorders.

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Published on: November 28, 2019

Area of Science:

Neuroscience
Urology
Molecular Biology

Background:

Understanding bladder afferent pathways is crucial for developing therapies for lower urinary tract disorders like overactive bladder and cystitis.
The transient receptor potential vanilloid 1 (TRPV1) ion channel is a potential candidate molecule involved in bladder function.
Previous studies suggest TRPV1 channels may influence the detection of bladder filling, impacting micturition thresholds.

Purpose of the Study:

To investigate the role of TRPV1 receptors in controlling bladder afferent sensitivity in mice.
To utilize pharmacological blockade and genetic deletion of TRPV1 to assess its function in bladder afferent pathways.

Main Methods:

Recorded multiunit afferent activity in vitro from bladder afferents of wild-type (TRPV+/+) and TRPV1 knockout (TRPV1-/-) mice.
Administered the TRPV1 antagonist capsazepine to wild-type preparations.
Assessed bladder afferent responses to mechanical distension, intravesical hydrochloric acid, and capsaicin in both genotypes.

Main Results:

Ramp bladder distension in wild-type mice produced a graded increase in afferent activity.
Capsazepine significantly attenuated afferent discharge in wild-type mice.
Afferent responses to distension were significantly attenuated in TRPV1-/- mice, with blunted sensitivity to acid and capsaicin. Low-threshold afferent responses were reduced in TRPV1-/- mice, while high-threshold sensitivity remained unchanged.

Conclusions:

TRPV1 channels play a significant role in the excitability of bladder afferents, especially low-threshold fibers.
The study demonstrates a clear role for TRPV1 in bladder afferent sensitivity, independent of changes in bladder compliance.
TRPV1 emerges as a potential therapeutic target for managing lower urinary tract disorders by modulating bladder afferent function.