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Related Experiment Video

Updated: Jul 13, 2026

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown.

Jeong Hoon Lee1, Seok Chung, Sung Jae Kim

  • 1Department of Electrical Engineering and Computer Science, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Analytical Chemistry
|July 14, 2007
PubMed
Summary

Researchers developed a novel nanofluidic preconcentrator using poly(dimethylsiloxane) (PDMS) microfluidic channels. This simple device achieves high protein concentration factors, advancing proteomics applications.

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Last Updated: Jul 13, 2026

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Published on: March 13, 2016

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Published on: December 2, 2011

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Area of Science:

  • Nanofluidics
  • Proteomics
  • Biotechnology

Background:

  • Efficient sample concentration is crucial for proteomics.
  • Existing methods often require complex fabrication.
  • Nanofluidic devices offer potential for sample enrichment.

Purpose of the Study:

  • To develop a simple and efficient nanofluidic preconcentrator.
  • To utilize the junction gap breakdown phenomenon for nanogap formation.
  • To demonstrate high protein preconcentration capabilities.

Main Methods:

  • Fabrication of a poly(dimethylsiloxane) (PDMS)-based microfluidic channel.
  • Formation of a nanogap via junction gap breakdown without photolithography.
  • Characterization of the nanogap using DC current measurements.
  • Quantification of beta-phycoerythrin protein preconcentration.

Main Results:

  • Successfully formed an approximately 80 nm deep nanogap.
  • Achieved a concentration volume of 70 pL for beta-phycoerythrin.
  • Obtained a concentration factor as high as 10^4 within 1 hour.
  • Demonstrated protein preconcentration across various buffers and pH values (5-9).

Conclusions:

  • The developed PDMS nanofluidic preconcentrator is simple and efficient.
  • The junction gap breakdown method offers a facile route to nanogap formation.
  • This technology significantly enhances protein preconcentration for proteomics applications.