The essential YycFG two-component system of Bacillus subtilis
Related Experiment Video
Updated: Jul 13, 2026

Using Coculture to Detect Chemically Mediated Interspecies Interactions
Published on: October 31, 2013
Hendrik Szurmant1, Tatsuya Fukushima, James A Hoch
1Department of Molecular and Structural Biochemistry, North Carolina State University, North Carolina, USA.
Abstract:
The YycFG two-component system, highly conserved in the low G+C gram positives, is essential for cell viability in most organisms in which it has been studied. The system is organized within an operon that includes at least one but often three to four other genes. Products of two of these genes, yycH and yycI, have been shown to have a regulatory role on this two-component system. Immunofluorescent studies identified YycG kinase localization at the cell division sites consistent with its role in regulating cell divisional processes. The essential nature and operon organization of this system commanded special requirements in studying this system genetically. This chapter presents methods utilized in identifying the regulatory circuit that controls the activity of the YycG kinase in Bacillus subtilis. Most aspects of our approaches are applicable to other two-component systems in B. subtilis and the gram positives. Some are limited to essential systems, such as the YycFG system.
More Related Videos
14:04Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Published on: February 1, 2010
12:23Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Published on: October 13, 2015
Related Concept Videos
Regulation of Bacterial Virulence
Bacterial Transformation
Bacterial Transformation
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Yeast Signaling
Gram-negative Bacterial Protein Secretion Systems