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Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier
Mihee Kim1, Chul-Ho Yun, Seong-Ki Park
1Department of Molecular Endocrinology, Chonnam National University, Gwangju, 500-757, Korea.
Abstract:
We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known as an epitope region, using a chemical cross-linker and injected into rabbits with adjuvant. By immunizing the animals intradermally twice at two week intervals, an immunoblot assay with anti-sera showed the efficient generation of the PAb.
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