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Nitropeptide Profiling and Identification Illustrated by Angiotensin II
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Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method

Serena Camerini1, Maria L Polci, Umberto Restuccia

  • 1San Raffaele Scientific Institute, 20132 Milan, Italy. serena.camerini@iss.it

Journal of Proteome Research
|July 17, 2007
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...