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Updated: Jul 13, 2026

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Published on: October 7, 2020
Classical and spermatocytic seminoma in the dog: histochemical and immunohistochemical findings
V Grieco1, E Riccardi1, M Rondena1
1Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione di Anatomia Patologica Veterinaria e Patologia Aviare, Facoltà di Veterinaria, Università degli Studi di Milano, Milano, Italy.
Abstract:
In the light of earlier human studies, 43 canine tumours diagnosed as seminoma were examined histologically with haematoxylin and eosin and periodic acid-Schiff (PAS) stains, and immunohistochemically with a monoclonal antibody against human placental alkaline phosphatase (PLAP). Twenty tumours were positive for both PAS and PLAP and were therefore diagnosed as classical seminoma (SE). The other 23 tumours were negative for both PAS and PLAP and were therefore diagnosed as spermatocytic seminoma (SS). Tubules with carcinoma in situ (CIS) were present in the testicular parenchyma surrounding 15 SEs and nine SSs.
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