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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA
Ping Wang1, Xiuting Li, Mufei Xiang
1Department of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, PR China. wangp@th.btbu.edu.cn
Journal of Bioscience and Bioengineering
|July 17, 2007
Summary
Activated sludge was domesticated to enrich aerobic denitrifiers using sequencing batch reactors (SBRs). Eight bacterial strains, including the novel aerobic denitrifier Delftia, were identified, offering new avenues for nitrogen removal.
Area of Science:
- Environmental microbiology
- Wastewater treatment technologies
Background:
- Aerobic denitrification is crucial for removing total inorganic nitrogen (TIN) from wastewater.
- Enrichment of specialized microbial communities is key to efficient aerobic denitrification.
Purpose of the Study:
- To domesticate activated sludge for aerobic denitrifier enrichment.
- To identify bacterial strains capable of aerobic denitrification.
Main Methods:
- Utilized two sequencing batch reactors (SBRs) under intermittent aeration.
- Isolated and identified bacteria using bromthymol blue (BTB) medium, PCR, and 16S rRNA gene sequencing.
- Analyzed sequences via BLAST search and phylogenetic tree construction.
Main Results:
- Achieved 60% total inorganic nitrogen (TIN) removal efficiency.
- Isolated 20 bacterial strains, with 8 identified belonging to genera Pseudomonas, Delftia, Herbaspirillum, and Comamonas.
- Identified Delftia as a potential novel aerobic denitrifier.
Conclusions:
- Successfully enriched aerobic denitrifiers from activated sludge.
- The identified bacterial genera, particularly Delftia, show promise for enhancing aerobic denitrification processes.
- Further research into Delftia's aerobic denitrification capabilities is warranted.
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