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Evaluation of disc susceptibility tests performed directly from positive blood cultures
1Division of Laboratory Medicine, Changi General Hospital, Singapore. thean_yen_tan@cgh.com.sg
Aims:
To evaluate the accuracy of direct disc susceptibility testing performed from positive BACTEC blood culture vials, using a predetermined dilution protocol.
Methods:
Direct susceptibility testing was performed from 432 positive blood culture vials, generating 3829 antibiotic-organism results. Results were compared with those obtained by standard disc susceptibility testing according to Clinical Laboratory Standards Institute (CLSI) methods.
Results:
When results were compared with the reference method, no very major errors were detected. One (0.03%) major error and 89 (2.3%) minor errors were found. Error rates by organism group ranged from 1.3% for Pseudomonas aeruginosa to 8.2% for beta-haemolytic streptococci.
Conclusions:
Direct susceptibility testing provided accurate susceptibility results for most organism-antibiotic combinations, with the exception of the beta-haemolytic streptococci.
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