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Related Experiment Video

Updated: Jul 13, 2026

Asymmetric Walkway: A Novel Behavioral Assay for Studying Asymmetric Locomotion

Asymmetric Walkway: A Novel Behavioral Assay for Studying Asymmetric Locomotion

Published on: January 15, 2016

Asymmetry in kinesin walking.

Qiang Shao¹, Yi Qin Gao

¹Department of Chemistry, Texas A&M University, College Station, Texas 77845, USA.

|July 17, 2007

Summary

Conventional kinesin 1

Area of Science:

Molecular Motor Dynamics
Biophysics
Cellular Transport

Background:

Kinesin 1's movement, a homodimer, is proposed to be an asymmetric hand-over-hand mechanism.
Previous research explored force-dependent features of kinesin's hand-over-hand motion.

Purpose of the Study:

Investigate the asymmetry in the kinesin 1 hand-over-hand mechanism.
Explain experimental observations like kinesin limping and processive motion in mutants.

Main Methods:

Computational modeling of kinesin stepping.
Analysis of kinesin 1's neck linker lengths variation.
Study of kinesin heterodimer and wild-type movement with ATP/AMPPNP.

Main Results:

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Kinesin limping is explained by variations in neck linker lengths during stepping.

Processive motion of a kinesin heterodimer is modeled.

A distinct force-generating kinesin-ATP complex intermediate is proposed for processive walking.

Conclusions:

Neck linker length variation is crucial for explaining kinesin 1's asymmetric movement and limping.
The kinesin-ATP complex intermediate differentiates processive walking from posthydrolytic states.