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Cryopreserved cells facilitate cell-based drug discovery.

Guido J R Zaman1, Jeroen A D M de Roos, Marion Blomenröhr

  • 1Molecular Pharmacology Unit, Organon BioSciences, Oss, The Netherlands. guido.zaman@organon.com

Drug Discovery Today
|July 17, 2007
PubMed
Summary

Cryopreserved cells enhance drug discovery by improving the consistency of cell-based functional assays. This method reduces variability and false positives in screening G protein-coupled receptors.

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Area of Science:

  • Pharmacology
  • Cell Biology
  • Drug Discovery

Background:

  • Cell-based functional assays are increasingly used in early drug discovery, especially for G protein-coupled receptors.
  • Live-cell assays offer advantages over lysed-cell assays by reducing false positives.

Purpose of the Study:

  • To highlight the benefits of using cryopreserved cells in drug discovery.
  • To demonstrate how cryopreservation improves the consistency and reliability of cell-based assays.

Main Methods:

  • Utilizing cryopreserved cells instead of continuously cultured cells.
  • Implementing live-cell screening assays.
  • Comparing results from cryopreserved versus cultured cells.

Main Results:

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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
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  • Cryopreservation decreases day-to-day variation in cell-based assay results.
  • The use of cryopreserved cells eliminates passage effects, leading to more consistent outcomes.
  • Live-cell assays with cryopreserved cells reduce the likelihood of false positives.

Conclusions:

  • Cryopreservation of cells is a valuable technique for improving the consistency and reliability of cell-based functional assays in drug discovery.
  • This method allows cells to be used as reagents, similar to enzymes, uncoupling cell culturing from screening activities.
  • Employing cryopreserved cells enhances the efficiency and accuracy of screening G protein-coupled receptors and other targets.