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Updated: Jul 13, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
Published on: March 19, 2012
H1.X with different properties from other linker histones is required for mitotic progression
Hideaki Takata1, Sachihiro Matsunaga, Akihiro Morimoto
1Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.
Abstract:
We report here the characterization of H1.X, a human histone H1 subtype. We demonstrate that H1.X accumulates in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. In addition, the results of fluorescence recovery after photobleaching indicate that the exchange of H1.X on and off chromatin is faster than that of the other H1 subtypes. Furthermore, RNA interference experiments reveal that H1.X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes.
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