Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural constraints on human immunodeficiency virus type 1 Nef function.

Alexa Raney1, Alice Y Shaw, John L Foster

  • 1University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.

Virology
|July 17, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The decay of HIV under anti-retroviral therapy is biphasic even in humanized mice with just T cells.

Journal of virology·2025
Same author

Combining spironolactone to antiretroviral therapy accelerates HIV decay in humanized mice.

Emerging microbes & infections·2025
Same author

SARS-CoV-2 infection induces pro-fibrotic and pro-thrombotic foam cell formation.

Nature microbiology·2025
Same author

Envelope-dimer epitope 1 (EDE1) antibody (C10) treatment significantly reduces Zika virus replication in the male and female reproductive tracts.

Journal of virology·2025
Same author

Humanized Mouse Systems to Study Viral Infection: A New Era in Immunology Research.

Annual review of immunology·2025
Same author

RSV infection of humanized lung-only mice induces pathological changes resembling severe bronchiolitis and bronchopneumonia.

Frontiers in virology (Lausanne, Switzerland)·2024
Same journal

Genomic and functional characterization of Bacillus phage BCE1 targeting a key gut bacterium in Aedes albopictus larvae.

Virology·2026
Same journal

Viral etiology of orogenital papillomatosis and squamous cell carcinoma in bottlenose dolphins in the southeastern United States.

Virology·2026
Same journal

Orthoflaviviruses in the modern era: Challenges and breakthroughs.

Virology·2026
Same journal

Post-COVID-19 immune dysregulation and autoimmune sequelae.

Virology·2026
Same journal

GenomoBase: A comprehensive resource for the family Genomoviridae.

Virology·2026
Same journal

Corrigendum to "The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing antibodies". [Virol. 509 [2017] 185-194].

Virology·2026
See all related articles

HIV-1 Nef protein function is complex. Tagging Nef

Area of Science:

  • Virology
  • Molecular Biology
  • Protein Biochemistry

Background:

  • HIV-1 Nef is a key viral protein.
  • Nef modulates host cell surface receptors (CD4, MHC-I) and activates PAK-2 kinase.
  • Nef's functional domains and interaction mechanisms are not fully understood.

Purpose of the Study:

  • To investigate the impact of N- and C-terminal tagging on HIV-1 Nef protein structure and function.
  • To elucidate the structural requirements for Nef's MHC-I down-modulation and PAK-2 activation activities.

Main Methods:

  • Genetic manipulation of HIV-1 Nef by inserting HA/FLAG and GFP tags at N- and C-termini.
  • Assessment of MHC-I down-modulation activity using cell-based assays.
  • Evaluation of PAK-2 kinase activation by Nef variants.

Related Experiment Videos

Main Results:

  • N- and C-terminal HA/FLAG tags significantly impaired MHC-I down-modulation.
  • C-terminal GFP tag resulted in defective PAK-2 activation.
  • Nef-GFP and Nef-GH(7) retained MHC-I down-modulating activity, suggesting tag charge influences function.

Conclusions:

  • Nef's flexible N- and C-terminal regions possess structural requirements for PAK-2 activation and MHC-I down-modulation.
  • Tagging strategies can reveal insights into protein structure-function relationships.
  • The negative charge of tags may interfere with specific Nef functions.