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Cellular microarrays for use with capillary-driven microfluidics.

Robert Lovchik1, Corinne von Arx, Angelika Viviani

  • 1IBM Research GmbH, Zurich Research Laboratory, 8803, Rüschlikon, Switzerland.

Analytical and Bioanalytical Chemistry
|July 17, 2007
PubMed
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We developed two methods for cell arraying in microfluidic capillary systems (CSs). The second method, using patterned poly(dimethylsiloxane) with fibronectin, enables precise single or multiple cell arrangement for high-throughput assays.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Microfluidic capillary systems (CSs) require efficient methods for cellular arraying.
  • Current techniques face challenges in controlling cell distribution and enabling high-throughput analysis.

Purpose of the Study:

  • To develop and compare two distinct methods for facile cell arraying on microstructured substrates.
  • To assess the suitability of these methods for cellular assays within microfluidic capillary systems.
  • To establish a versatile platform for high-throughput, single-cell level experimentation.

Main Methods:

  • Microcontact printing of alkanethiols on silicon substrates to create adhesive fibronectin patterns for cell attachment.
  • Utilizing poly(dimethylsiloxane) elastomers with recessed microstructures for selective fibronectin and bovine serum albumin (BSA) coating.

Related Experiment Videos

  • Arraying human breast cancer cells based on patterned surface chemistry and microstructure geometry.

    • Main Results:

    • The first method, using alkanethiols, resulted in cellular clusters that were difficult to control.
    • The second method, employing patterned poly(dimethylsiloxane), successfully enabled controlled arraying of single or multiple cells.
    • The size of the microstructures influenced the number of cells arrayed.

    Conclusions:

    • Patterned poly(dimethylsiloxane) offers a superior approach for controlled cell arraying in microfluidic systems.
    • This technique facilitates high-throughput experimentation at the single-cell level.
    • The developed method provides a versatile platform for advanced cellular assays.