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Updated: Jul 13, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
[Isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis]
Yun-Duan Wang1, Xiao-Jun Dong, Xi Wang
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Abstract:
Five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. They were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. Strain PHD-2, PHD-4 and PHD-5 belonged to the genera of Ralstonia, Acinetobacter and Microbacterium respectively; strain PHD-1 and PHD-3 were from the genus of Pseudomonas. Homology comparing of their 16S rRNA gene sequences and phylogenetic analysis displayed the high biodiversity of phenol-degrading microorganisms in the natural soil.

