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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Identifying genes that extend life span using a high-throughput screening system.

Cuiying Chen1, Roland Contreras

  • 1Department of Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology (VIB), Ghent, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|July 20, 2007
PubMed
Summary

Researchers developed a novel screening system to identify genes that extend lifespan in yeast. This method isolates long-lived mother cells based on their bud scar count, enabling genetic discovery for aging research.

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Area of Science:

  • Genetics
  • Cell Biology
  • Aging Research

Background:

  • Determining genes that influence lifespan is crucial for understanding aging.
  • Baker's yeast (Saccharomyces cerevisiae) is a model organism for aging studies.
  • Identifying long-lived cells is a key challenge in functional genomic screening.

Purpose of the Study:

  • To develop a high-throughput screening system for identifying lifespan-extending genes in yeast.
  • To isolate and analyze yeast mother cells exhibiting extended longevity.
  • To leverage bud scar counting as a proxy for cellular lifespan.

Main Methods:

  • Developed a functional genomic screening system using Saccharomyces cerevisiae.
  • Utilized fluorescently labeled wheat germ agglutinin (WGA) to stain chitin bud scars.
  • Employed microbeads for successive purification and regrowth of mother cells (M-cells).
  • Applied flow cytometry to sort and isolate cells with a high number of bud scars, indicative of longer lifespan.

Main Results:

  • Successfully established a system for isolating long-lived yeast mother cells.
  • Demonstrated the utility of bud scar number, visualized by WGA staining, as a lifespan indicator.
  • Enabled high-throughput identification of genes associated with prolonged cellular lifespan.

Conclusions:

  • The developed bud-scar-sorting system is effective for identifying lifespan-extending genes in yeast.
  • This methodology provides a powerful tool for aging research and functional genomics.
  • The system allows for efficient isolation of long-lived cells for further genetic analysis.