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Related Concept Videos

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
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Suppression Subtractive Hybridization (SSH) and its modifications in microbiological research.

Xiaowei Huang1, Yunxia Li, Qiuhong Niu

  • 1Lab for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, China.

Applied Microbiology and Biotechnology
|July 20, 2007
PubMed
Summary

Suppression subtractive hybridization (SSH) identifies gene expression changes. This review highlights SSH applications for detecting functional genes and DNA markers in microbial genomes.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Microbiology

Background:

  • Suppression subtractive hybridization (SSH) is a key technique for identifying differentially expressed genes.
  • It is widely applied in higher eukaryotes to understand complex diseases like cancer.
  • Microbial genomics offers unique applications for SSH due to smaller genome sizes.

Purpose of the Study:

  • To review Suppression Subtractive Hybridization (SSH) and its modified techniques.
  • To focus on the application of SSH in detecting specific functional genes and DNA markers in microorganisms.
  • To explore SSH's utility in microbial genomics and diversity studies.

Main Methods:

  • Review of existing literature on Suppression Subtractive Hybridization (SSH).
  • Analysis of SSH modifications and their specific applications.
  • Focus on SSH utility in microbial contexts, including mRNA and genomic DNA analysis.

Main Results:

  • SSH effectively identifies genes with varying expression levels in biological processes.
  • SSH and its modifications are valuable for isolating specific chromosomal loci in microbes.
  • The technique aids in studying genomic diversity linked to microbial secondary metabolism and specialized functions.

Conclusions:

  • SSH is a versatile tool for gene discovery in both eukaryotes and prokaryotes.
  • Its application in microbiology extends beyond mRNA analysis to genomic feature identification.
  • SSH provides insights into microbial genomic diversity and functional gene content.