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Related Experiment Video

Updated: Jul 13, 2026

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Tissue microarray is inappropriate for analysis of BCL6 expression in diffuse large B-cell lymphoma.

Johan Linderoth1, Mats Ehinger, Måns Akerman

  • 1Department of Oncology, Lund University Hospital, Lund, Sweden. johan.linderoth@med.lu.se

European Journal of Haematology
|July 20, 2007
PubMed
Summary

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Tissue microarrays (TMA) are unreliable for classifying diffuse large B-cell lymphoma (DLBCL) cell-of-origin due to BCL6 heterogeneity. This impacts patient survival outcomes and treatment decisions.

Area of Science:

  • Hematology
  • Oncology
  • Pathology

Background:

  • Accurate classification of diffuse large B-cell lymphoma (DLBCL) into germinal center (GC) and non-GC subtypes is crucial for treatment.
  • Immunohistochemistry (IHC) is proposed for routine cell-of-origin classification.
  • Technical standardization is needed before routine implementation.

Purpose of the Study:

  • To evaluate the influence of different immunohistochemical techniques on BCL6 positivity and GC/non-GC categorization in DLBCL.
  • To assess the reliability of tissue microarray (TMA) versus whole tissue sections (WTS) for DLBCL subtyping.

Main Methods:

  • 122 patients with de novo stage II-IV DLBCL were studied.
  • Immunohistochemical expression of BCL6, CD10, and MUM-1/IRF4 was analyzed using TMA and WTS.

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Last Updated: Jul 13, 2026

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07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

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  • BCL6 and CD10 were assessed on both TMA and WTS.

    • Main Results:

    • BCL6 showed significant heterogeneity and high false-negative rates with TMA (25%) compared to WTS (53%).
    • CD10 expression was more homogeneous, with better concordance between TMA and WTS.
    • GC/non-GC DLBCL categorization differed significantly between TMA and WTS, impacting overall survival predictions.

    Conclusions:

    • Immunohistochemical GC-status determination using TMA is not sufficiently reliable for individual treatment decisions in DLBCL.
    • Difficulties in interpreting BCL6 status on TMA are a primary limitation.
    • Further technical optimization is required for reliable DLBCL subtyping.