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Related Experiment Videos

Vascular proteomic mapping in vivo.

A B Simonson1, J E Schnitzer

  • 1Sidney Kimmel Cancer Center, San Diego, CA 92121, USA.

Journal of Thrombosis and Haemostasis : JTH
|August 1, 2007
PubMed
Summary
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Accelerating drug development requires new methods to validate molecular targets. This review highlights advances in proteomic imaging for discovering and validating vascular targets in vivo.

Area of Science:

  • Biomedical research
  • Molecular biology
  • Medical imaging

Background:

  • Molecular targeting is crucial but challenging in medicine.
  • Genomics and proteomics identify disease-related targets, but in vivo validation is slow.
  • A need exists for integrated approaches to accelerate target validation.

Purpose of the Study:

  • To review vascular targeting and proteomics.
  • To present recent developments in proteomic imaging.
  • To discuss a novel in vivo organellar proteomic imaging platform.

Main Methods:

  • Subcellular fractionation
  • Mass spectrometry
  • Bioinformatic database interrogation
  • Monoclonal antibody technology

Related Experiment Videos

  • In vivo imaging modalities

    • Main Results:

    • Development of an in vivo organellar proteomic imaging platform.
    • Discovery of new organ-specific vascular targets.
    • Improved standards for in vivo target validation.
    • Advancements in tumor imaging and radioimmunotherapy.

    Conclusions:

    • Technological progress enables large-scale proteomic mapping and target discovery.
    • New targets facilitate organ-specific in vivo targeting.
    • Imaging improvements enhance target validation in vivo.
    • Further research is needed for comprehensive endothelial protein mapping.