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Related Concept Videos

Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

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Related Experiment Video

Updated: Jul 13, 2026

Metabolic Profile Analysis of Zebrafish Embryos
05:41

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Published on: January 14, 2013

Murine pre-embryo oxygen consumption and developmental competence.

Lars D M Ottosen1, Johnny Hindkjaer, Svend Lindenberg

  • 1The Fertility Clinic, Department of Obstetrics and Gynaecology, Aarhus University Hospital Skejby Sygehus, Brendstrupgaardsvej, 8200, Arhus N, Denmark. lars.ottosen@ki.au.dk

Journal of Assisted Reproduction and Genetics
|July 20, 2007
PubMed
Summary

Investigating oxygen consumption in early mouse embryos, this study found that high oxygen consumption in 4-cell embryos may indicate better development to the blastocyst stage. However, it is not a strong predictor for embryo viability.

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Area of Science:

  • Reproductive biology
  • Embryology
  • Metabolic research

Background:

  • Preimplantation embryo viability is crucial for successful implantation and pregnancy.
  • Identifying reliable markers for embryo development is essential in assisted reproductive technologies.
  • Oxygen consumption is a key metabolic indicator in cellular processes.

Purpose of the Study:

  • To investigate oxygen consumption in individual murine cleavage-stage embryos.
  • To evaluate the predictive value of oxygen consumption for subsequent development to the expanded blastocyst stage.
  • To explore oxygen consumption as a potential marker for preimplantation embryo viability.

Main Methods:

  • 248 murine embryos were monitored from the 2-cell to blastocyst stage.
  • Individual oxygen consumption was measured at different developmental stages.
  • Embryos were grouped by oxygen consumption levels to calculate odds ratios for blastocyst development.

Main Results:

  • Oxygen consumption increased significantly from the cleavage stage (0.16-0.20 nl O(2) h(-1)) through the morula stage (0.21-0.23 nl O(2) h(-1)) to the expanded blastocyst stage (0.47 nl O(2) h(-1)).
  • Four-cell embryos with high oxygen consumption showed a significantly higher chance of developing into expanded blastocysts (OR 2.25).
  • No significant difference in blastocyst development was observed between high and low oxygen-consuming 2-cell embryos. The study's method slightly reduced the blastocyst rate (51% vs. 80% in controls).

Conclusions:

  • Oxygen consumption in cleavage-stage embryos may serve as an indicator of subsequent development.
  • Oxygen consumption is not yet a strong predictor of expanded blastocyst development.
  • Further research is needed to validate oxygen consumption as a reliable marker for embryo viability, considering potential methodological impacts.