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Updated: Jul 13, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
Published on: October 17, 2025
Application of bioinformatics resources for genosensor design
Tamara M Khlebodarova1, Nina V Tikunova, Alla V Kachko
1Institute of Cytology and Genetics SB RAS, Lavrentieva Ave., 10, Novosibirsk 630090, Russia. tamara@bionet.nsc.ru
Abstract:
Two novel databases, GenSensor and ConSensor, have been developed. GenSensor accumulates information on the sensitivities of the prokaryotic genes to external stimuli and may facilitate designing of novel genosensors; ConSensor contains data about the structure and efficiency of the available genosensor plasmid constructs. Using these databases, candidate genes for the design of novel multiple functional genosensors were searched, and the Escherichia coli dps gene was chosen as the candidate. The genetic construct derived from its promoter was developed and tested for its sensitivity to various stress agents: hydrogen peroxide (oxidative stress), phenol (protein and membrane damaging), and mitomycin C (DNA damaging). This genosensor was found to be sensitive to all stress conditions applied confirming its ability to serve as multi-functional genosensor. The GenSensor and ConSensor databases are available at http://wwwmgs.bionet.nsc.ru/mgs/dbases/gensensor/index.html.
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