Related Experiment Video
Updated: Jul 13, 2026
09:22
Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
Published on: September 3, 2010
Photocleavable molecule for laser desorption ionization mass spectrometry
1Joint Research Center, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. maki@nagasaki-u.ac.jp
The Journal of Organic Chemistry
|July 20, 2007
Summary
A novel photocleavable molecule enhances laser desorption ionization mass spectrometry (LDI-MS) detection sensitivity and stability. This new molecular tag enables kinetic studies of chemical reactions like amidation and aminolysis.
Area of Science:
- Analytical Chemistry
- Organic Chemistry
- Mass Spectrometry
Background:
- Laser desorption ionization mass spectrometry (LDI-MS) is a powerful analytical technique.
- Development of sensitive and stable molecular tags is crucial for advanced LDI-MS applications.
- Photocleavable molecules offer unique advantages for controlled tagging and release.
Purpose of the Study:
- To design and synthesize a novel photocleavable molecule for LDI-MS.
- To evaluate the molecule's performance in terms of sensitivity and chemical stability.
- To demonstrate its utility as a molecular tag for kinetic measurements.
Main Methods:
- Chemical synthesis of the photocleavable molecule.
- Characterization using mass spectrometry (MS).
- Application as a molecular tag in kinetic studies of amidation and aminolysis reactions.
Main Results:
- Successful design and synthesis of a new photocleavable molecule.
- Demonstrated high sensitivity for negative mode MS detection.
- Exhibited good chemical stability for practical applications.
- Successfully applied as a molecular tag in LDI-MS.
Conclusions:
- The new photocleavable molecule is a promising tool for sensitive LDI-MS analysis.
- Its stability and tagging capability facilitate kinetic studies of important chemical transformations.
- This development advances the application of LDI-MS in chemical reaction monitoring.
Related Concept Videos
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
01:19Mass Spectrometry: Overview
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
01:21Mass Spectrometry: Complex Analysis
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
01:20Mass Spectrometry: Molecular Fragmentation Overview
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
01:16Mass Spectrometers
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions: