Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Delineating v-Src downstream effector pathways in transformed myoblasts.

L Ciuffini1, L Castellani, E Salvati

  • 1Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Monterotondo Scalo (RM), Italy.

Oncogene
|July 20, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multidisciplinary working group: key role for percutaneous endovascular AV fistula program. Point of view.

Journal of nephrology·2023
Same author

PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment.

Communications biology·2023
Same author

A closer look to the new frontier of artificial intelligence in the percutaneous treatment of primary lesions of the liver.

Medical oncology (Northwood, London, England)·2020
Same author

A systematic review and Meta-analysis on the association between Hand-Foot Syndrome (HFS) and Cancer Chemotherapy Efficacy.

La Clinica terapeutica·2019
Same author

Multiscale analysis of bamboo deformation mechanisms following NaOH treatment using X-ray and correlative microscopy.

Acta biomaterialia·2018
Same author

Strain softening of nano-scale fuzzy interfaces causes Mullins effect in thermoplastic polyurethane.

Scientific reports·2017
Same journal

Correction: Neuropilin-1 promotes human glioma progression through potentiating the activity of the HGF/SF autocrine pathway.

Oncogene·2026
Same journal

Amphiregulin-mediated EGFR activation drives both intrinsic and acquired resistance to KRAS G12C inhibitors in KRAS G12C-mutant non-small cell lung cancer.

Oncogene·2026
Same journal

Histone lactylation-driven IGF2BP3 promotes intrahepatic cholangiocarcinoma progression via SPP1/CD44-dependent macrophage polarization.

Oncogene·2026
Same journal

Correction: SIRT7 activates p53 by enhancing PCAF-mediated MDM2 degradation to arrest the cell cycle.

Oncogene·2026
Same journal

Correction: Liver-specific SIRT1 knockout-induced hyperglycemia promotes spontaneous lung adenocarcinomas through HSF1-MDM2.

Oncogene·2026
Same journal

Correction: 6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells.

Oncogene·2026
See all related articles

v-Src tyrosine kinase drives avian myoblast proliferation by inhibiting p38 MAPK. Restoring p38 MAPK activity and inhibiting MAPK kinase can reverse these effects, enabling muscle differentiation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • v-Src tyrosine kinase is implicated in cellular transformation.
  • Understanding signaling pathways is crucial for targeting cancer growth and differentiation.

Purpose of the Study:

  • To investigate intracellular signaling pathways affected by v-Src in avian myoblasts.
  • To identify key targets for reversing v-Src-induced proliferation and blocked differentiation.

Main Methods:

  • Inhibition of Ras-MAPK kinase and phosphatidylinositol 3-kinase.
  • Forced activation of p38 MAPK.
  • Analysis of muscle-specific gene expression and cell fusion.

Main Results:

  • Both Ras-MAPK kinase and PI3K pathways are essential for v-Src-mediated proliferation.

Related Experiment Videos

  • Ras-MAPK kinase pathway blocks differentiation independently of proliferation.
  • v-Src inhibits p38 MAPK; its activation inhibits growth and promotes differentiation.
  • Cross-regulation between Ras-MAPK kinase and p38 MAPK pathways observed.

    • Conclusions:

    • p38 MAPK is a critical target for v-Src-induced growth transformation and blocked myogenic differentiation.
    • Targeting MAPK kinase and calpain, alongside p38 MAPK activation, can restore differentiation potential in v-Src-transformed myoblasts.