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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
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Reporting data from high-throughput screening of small-molecule libraries.

James Inglese1, Caroline E Shamu, R Kiplin Guy

  • 1US National Institutes of Health Chemical Genomics Center, National Human Genome Institute, National Institutes of Health, 9800 Medical Center Drive, Bethesda, Maryland 20892-3370, USA. jinglese@mail.nih.gov

Nature Chemical Biology
|July 20, 2007
PubMed
Summary
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Standardized reporting guidelines are needed for small-molecule screening data from high-throughput screening (HTS) studies. This work proposes concise guidelines to improve the quality and interpretability of published HTS results.

Area of Science:

  • Drug discovery and development
  • Chemical biology
  • Biotechnology

Background:

  • High-throughput screening (HTS) is increasingly utilized in academic research for small-molecule discovery.
  • Current publications often lack standardized reporting for small-molecule screening data, hindering evaluation.
  • Key information for interpreting HTS results is frequently omitted in published protocols.

Purpose of the Study:

  • To propose concise guidelines for reporting small-molecule screening data.
  • To enhance the quality and reproducibility of published high-throughput screening results.
  • To facilitate better evaluation and interpretation of small-molecule screening data.

Main Methods:

  • Literature review of existing reporting practices in HTS.

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  • Development of a consensus-based set of reporting recommendations.
  • Focus on essential data elements for small-molecule screening.

    • Main Results:

    • Identification of critical information gaps in current HTS reporting.
    • Proposal of specific, actionable guidelines for data reporting.
    • Guidelines cover assay conditions, data analysis, and hit validation.

    Conclusions:

    • Adoption of standardized reporting guidelines will improve the scientific rigor of HTS publications.
    • Clearer reporting facilitates data sharing, collaboration, and downstream research.
    • These guidelines aim to enhance the utility and impact of small-molecule screening efforts.