Related Experiment Video
Updated: Jul 13, 2026
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
Published on: October 29, 2020
Antibacterial effects of a monoporphyrinato ytterbium(III) complex and its free components on Staphylococcus aureus
1Department of Chemistry, Wuhan University, Wuhan 430072, PR China. houanxin@yahoo.com
Abstract:
The antibacterial effect of Yb3+, the free porphyrin base 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin (H2TMP; 1), and the corresponding Yb3+ porphyrinato complex [Yb(III)(TMP)(H2O)3]+ Cl- (Yb(TMP); 2) towards Staphylococcus aureus was investigated by stop-flow microcalorimetry. By analyzing the obtained metabolic thermogenic curves, crucial parameters such as rate constant of bacterial growth (k), half inhibitory concentration (IC50), and generation time (t(G)) were determined. The antibacterial activities of the three compounds tested was 2>1>Yb3+, with an IC50 value of 273 mg/l for complex 2. The Yb3+ porphyrinato complex is proposed to benefit from synergetic effects of Yb3+ and the free porphyrin 1.
