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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Updated: Jul 13, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Microdissecting the proteome.

Ferdinand von Eggeling1, Christian Melle, Günther Ernst

  • 1Core Unit Chip Application, Institute of Human Genetics and Anthropology, Medical Faculty at the Friedrich Schiller University Jena, Jena, Germany. fegg@mti.uni-jena.de

Proteomics
|July 20, 2007
PubMed
Summary

Proteomic analysis requires separating distinct tissue regions before study. Laser microdissection enables this separation, ensuring accurate protein analysis and reliable results for diverse tissues.

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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Area of Science:

  • Proteomics
  • Molecular Biology
  • Biotechnology

Background:

  • The proteome's complexity necessitates spatial separation of tissues for accurate analysis.
  • Variations in protein composition across organs and within tissues can confound proteomic studies.
  • Ensuring that proteomic results reflect true tissue functions is critical.

Purpose of the Study:

  • To review the compatibility of microdissected tissues with various proteomic techniques.
  • To highlight the importance of tissue separation in proteomic research.
  • To assess the suitability of laser microdissection for proteomic sample preparation.

Main Methods:

  • Laser-based microdissection for tissue separation.
  • Analysis of formalin-fixed and cryopreserved tissues.
  • Evaluation of compatibility with 2-DE (two-dimensional gel electrophoresis), MS (mass spectrometry), and protein arrays.

Main Results:

  • Laser microdissection effectively separates functional tissue areas.
  • Microdissected tissues demonstrate compatibility with standard proteomic workflows.
  • The method preserves tissue integrity for subsequent proteomic analysis.

Conclusions:

  • Laser microdissection is a crucial technique for accurate proteomic studies.
  • Proper tissue separation ensures the reliability and validity of proteomic data.
  • This approach enhances the ability to study tissue-specific protein expression.