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Updated: Jul 13, 2026
Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
Published on: October 9, 2021
[Influence of tryptophan content in proteins on the quantitative determination of this proteins using photochemical
Abstract:
The interaction of proteins containing various number of Trp residues with p-dimethylaminobenzaldehyde (pDABA) was studied. A positive correlation between tryptophan content in proteins and absorbance of protein-pDABA reaction products was found (Spearman's correlation coefficient R = 0.936; p < 0.001). Using the observed correlation we were able to predict a possible range for the absorbance of reaction products for each serum globulin and estimate the contribution of different globulins in the pDABA serum assay. We propose that the described method can be used for the determination of the globulin content in blood serum.
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