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Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
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[Helicobacter pylori detected in atheroma plaque]

G Ghirardi1, F Maldonado, L Guzmán

  • 1Servicio de Anatomía Patológica y Departamento de Cardiologia, Hospital Córdoba, Córdoba, Argentina.

Revista De La Facultad De Ciencias Medicas (Cordoba, Argentina)
|July 21, 2007
PubMed

Insights

Helicobacter pylori infection was detected in over 40% of atherosclerotic plaques, suggesting a link between this bacterium and atherosclerosis. This finding highlights the potential role of H. pylori in cardiovascular disease development.

Area of Science:

  • Cardiovascular Pathology
  • Infectious Disease Research
  • Immunohistochemistry

Background:

  • Atherosclerosis and coronary illness (CI) are often linked to chronic inflammation and infection.
  • Previous studies suggest a correlation between Helicobacter pylori infection and CI, particularly with virulent strains.

Purpose of the Study:

  • To immunohistochemically detect the presence of H. pylori in atherosclerotic plaques from various vascular regions.
  • To investigate the association between H. pylori and inflammatory cells within these plaques.

Main Methods:

  • Analyzed 34 atherosclerotic plaques (25 male, 9 female) using immunohistochemistry (IHC).
  • Tissues underwent formalin fixation, formic acid decalcification (if needed), paraffin embedding, and H&E staining.
  • Employed a biotin-streptavidin-HRP-DAB IHC system with citrate-based antigen retrieval for H. pylori detection.

Main Results:

  • Helicobacter pylori was identified in 14 out of 34 cases (41.17%).
  • The detected bacteria were associated with chronic inflammatory cell infiltration within the plaques.
  • Bacterial presence was observed in both the vascular wall and lumen.

Conclusions:

  • The findings demonstrate the presence of H. pylori in a significant proportion of atherosclerotic plaques.
  • This suggests a potential role for H. pylori infection in the pathogenesis of atherosclerosis.
  • Further research is warranted to elucidate the mechanisms linking H. pylori to cardiovascular disease.
Abstract

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