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Carbohydrate storage in anaerobic sequencing batch reactors.

Toshio Shimada1, Julie Zilles, Lutgarde Raskin

  • 1University of Illinois, Urbana-Champaign, 205 N. Mathews Ave., IL 61801, USA.

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Trehalose accumulates and degrades as a storage compound in glucose-fed anaerobic sequencing batch reactors (ASBRs). This intracellular carbohydrate production explains organic matter dynamics during the 24-hour cycle.

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Area of Science:

  • Biochemistry
  • Environmental Microbiology
  • Chemical Engineering

Background:

  • Anaerobic sequencing batch reactors (ASBRs) are crucial for organic waste treatment.
  • Understanding microbial storage compounds is key to optimizing ASBR performance.
  • Trehalose's role in anaerobic digestion has not been fully elucidated.

Purpose of the Study:

  • To investigate the accumulation and degradation of trehalose in glucose-fed ASBRs.
  • To explain the transient organic matter dynamics observed during ASBR cycles.
  • To modify and validate a mathematical model for ASBRs incorporating microbial storage.

Main Methods:

  • Fed glucose to an ASBR and monitored organic matter (COD) and methane production.
  • Performed total carbohydrate analysis to quantify accumulated carbohydrates.
  • Utilized 13C-nuclear magnetic resonance (NMR) to identify the specific carbohydrate.
  • Modified the IWA Anaerobic Digestion Model No. 1 (ADM1) to include microbial storage.

Main Results:

  • 40% of influent COD was initially unaccounted for by methane and soluble COD.
  • 26% of added glucose transiently accumulated as intracellular carbohydrate within the biomass.
  • Trehalose was identified as the primary storage carbohydrate using 13C-NMR.
  • The modified ADM1 model accurately described ASBR dynamics with microbial storage.

Conclusions:

  • Trehalose accumulation and degradation explain the observed organic matter fluctuations in ASBRs.
  • Incorporating microbial storage into models like ADM1 improves the prediction of ASBR performance.
  • This study provides insights into microbial metabolism for enhanced anaerobic digestion efficiency.