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Virus-like particles in geometric tubuloreticular structures.
Journal of the National Cancer Institute
|April 1, 1976
Summary
Geometric tubuloreticular structures were found in dog osteosarcoma cells. After passage through mice, these structures contained virus-like particles, suggesting a murine origin for the aberrant type-C particles.
Area of Science:
- Cell Biology
- Virology
- Oncology
Background:
- Tubuloreticular structures (TRS) are observed in various cell types and conditions.
- Canine osteosarcoma is a common bone cancer in dogs.
- The origin and nature of virus-like particles (VLPs) in cell cultures can be complex.
Purpose of the Study:
- To investigate the presence and characteristics of tubuloreticular structures in dog osteosarcoma cells.
- To determine the potential influence of cocultivation with human placenta cells on these structures.
- To examine the emergence of virus-like particles following in vivo passage in NIH nude mice.
Main Methods:
- Dog osteosarcoma cells (D17a) were cultured and cocultivated with human placenta cells.
- Cocultivated cells were passaged through NIH nude mice.
- Electron microscopy was used to examine cellular structures and virus-like particles.
Main Results:
- Geometric tubuloreticular structures were observed in D17a cells before and after cocultivation.
- After passage through NIH nude mice, reestablished cultures produced conventional murine type-C particles.
- Budding virus-like particles (VLPs) were observed within the tubuloreticular structures in post-mouse passage cultures.
Conclusions:
- Virus-like particles observed in dog osteosarcoma cells after mouse passage are likely of murine origin.
- The tubuloreticular structures may serve as a site for the assembly or display of these aberrant type-C particles.
- This study provides insights into viral particle formation in osteosarcoma cells under specific experimental conditions.