Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple forms of bFGF: differential nuclear and cell surface localization.

R Z Florkiewicz1, A Baird, A M Gonzalez

  • 1Department of Biochemistry, Whittier Institute for Diabetes and Endocrinology, La Jolla, CA 92037.

Growth Factors (Chur, Switzerland)
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physicochemical and functional characterization of the mucilage obtained from cladodes of two Opuntia species.

International journal of biological macromolecules·2024
Same author

Dualism, allosteric modulation, and biased signaling of opioid receptors: Future therapeutic potential.

Revista espanola de anestesiologia y reanimacion·2023
Same author

The relevance of false positive acid phosphatase reactions indicative of the presence of seminal fluid from oral and vaginal samples.

Science & justice : journal of the Forensic Science Society·2023
Same author

Homogeneous colleters in Myrteae (Myrtaceae).

Protoplasma·2023
Same author

Oromandibular-limb hypogenesis syndromes (OLHS): A challenge for airway management.

Revista espanola de anestesiologia y reanimacion·2021
Same author

Analysis of the care management protocol for COVID pregnant women and detection of improvement proposals applying clinical simulation methodology.

Revista espanola de anestesiologia y reanimacion·2020

Human basic fibroblast growth factor (bFGF) gene produces multiple proteins. Different initiation codons direct these bFGF isoforms to distinct cellular locations, impacting their functions.

Area of Science:

  • Molecular Biology
  • Cell Biology

Background:

  • The human basic fibroblast growth factor (bFGF) gene encodes multiple protein isoforms.
  • The function of larger bFGF isoforms remains largely unknown compared to the well-studied 18 kD isoform.

Purpose of the Study:

  • To compare the synthesis, transport, processing, and intracellular localization of individual bFGF isoforms.
  • To investigate how different translation initiation codons influence bFGF isoform localization and function.

Main Methods:

  • cDNA mutagenesis to alter translation initiation codons.
  • COS cell expression experiments to analyze protein synthesis and localization.
  • Differential localization studies using cell surface and nuclear markers.

Main Results:

Related Experiment Videos

  • Specific translation initiation codons (CUG or AUG) direct the synthesis of distinct bFGF isoforms.
  • Larger isoforms (24, 23, 22 kD) synthesized via CUG initiation are localized to the nucleus.
  • The 18 kD isoform, synthesized via AUG initiation, is exported to the cell surface but not secreted.

Conclusions:

  • Differential localization of bFGF isoforms is mediated by specific translation initiation codons.
  • bFGF isoforms exhibit distinct intracellular targeting, suggesting diverse functional roles.
  • Further research is needed to identify the targeting signals or cellular components responsible for bFGF localization.