Related Experiment Video

Updated: Jul 13, 2026

Forward Genetic Approaches in Chlamydia trachomatis
09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

Relative susceptibility of six continuous cell lines for cultivation of Chlamydia trachomatis strains

J Malathi1, G Shyamala, H N Madhavan

  • 1L & T Microbiology Research Center, Vision Research Foundation, Sankara Nethralaya, Chennai - 600 006, Tamil Nadu, India.

Indian Journal of Medical Microbiology
|July 24, 2007
PubMed

Insights

McCoy and HeLa cell lines are highly susceptible for Chlamydia trachomatis cultivation. Other cell lines like A549 showed no susceptibility, while Vero, BHK-21, and HEp-2 varied in their response to C. trachomatis infection.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Cell line susceptibility is crucial for efficient Chlamydia trachomatis cultivation.
  • Understanding host cell interactions is key to studying Chlamydia pathogenesis.

Purpose of the Study:

  • To evaluate the susceptibility of various cell lines to Chlamydia trachomatis infection.
  • To identify optimal cell lines for Chlamydia trachomatis research and propagation.

Main Methods:

  • Six cell lines (McCoy, HeLa, BHK-21, HEp-2, Vero, A549) were inoculated with C. trachomatis serovars A, B, Ba, and C.
  • Infection-forming units (IFUs) were quantified per microscopic field (100X) to assess growth.
  • Susceptibility was graded based on the observed IFU counts.

Main Results:

  • A549 cell line demonstrated no susceptibility to any tested C. trachomatis serovars.
  • McCoy and HeLa cell lines exhibited good to very good susceptibility.
  • Vero, BHK-21, and HEp-2 cell lines displayed variable susceptibility.

Conclusions:

  • McCoy and HeLa cell lines are recommended for Chlamydia trachomatis cultivation due to high susceptibility.
  • Cell line choice significantly impacts Chlamydia trachomatis propagation efficiency.
  • Further research may explore the specific mechanisms of susceptibility in different cell lines.

More Related Videos

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic of...
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...