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Plasmid mediated amikacin resistance in clinical isolates of Pseudomonas aeruginosa

M Shahid1, A Malik

  • 1Department of Microbiology, JN Medical College, Aligarh Muslim University, Aligarh - 202 002, India.

Indian Journal of Medical Microbiology
|July 24, 2007
PubMed

Insights

Multidrug resistant Pseudomonas aeruginosa strains from burn patients carry R plasmids. Plasmid analysis confirmed amikacin resistance is mediated by these plasmids, highlighting the need for characterization and policy changes.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Hospitalized burn patients are susceptible to infections.
  • Pseudomonas aeruginosa is a common cause of hospital-acquired infections.
  • Multidrug resistance (MDR) in bacteria poses a significant public health threat.

Purpose of the Study:

  • To investigate the role of plasmids in multidrug resistance in Pseudomonas aeruginosa.
  • To identify specific drug resistance mechanisms mediated by plasmids.
  • To provide recommendations for controlling the emergence of antibiotic resistance.

Main Methods:

  • Isolation and characterization of MDR Pseudomonas aeruginosa from burn patients.
  • Plasmid detection, curing, and transformation experiments.
  • Testing for beta-lactamase production.

Main Results:

  • All ten MDR Pseudomonas aeruginosa isolates harbored R plasmids.
  • Plasmid-mediated resistance to amikacin was confirmed.
  • Beta-lactamase production was also observed.

Conclusions:

  • R plasmids play a crucial role in the multidrug resistance of Pseudomonas aeruginosa.
  • Characterization of plasmids in MDR strains is essential.
  • A national antibiotic policy is recommended to mitigate the emergence of drug resistance.

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