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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

A study of diarrhoea among children in eastern Nepal with special reference to rotavirus

M Shariff1, M Deb, R Singh

  • 1Departments of Microbiology, BP Koirala Institute of Health Sciences, Dharan, Nepal.

Indian Journal of Medical Microbiology
|July 24, 2007
PubMed

Insights

Rotavirus infection affects nearly 40% of young children with acute gastroenteritis in Nepal. Enzyme immunoassay (EIA) is a more sensitive diagnostic tool than latex agglutination (LA) for detecting Rotavirus antigen.

Area of Science:

  • Pediatrics
  • Virology
  • Infectious Diseases

Background:

  • Rotavirus is a leading cause of severe diarrheal disease in infants and young children globally.
  • Gastroenteritis in children under five years of age poses a significant public health challenge.
  • Understanding the incidence of specific viral pathogens is crucial for effective disease management.

Purpose of the Study:

  • To determine the incidence of Rotavirus infection in children under five years of age presenting with acute gastroenteritis.
  • To evaluate diagnostic methods for Rotavirus antigen detection in faecal samples.

Main Methods:

  • Faecal samples were collected from 160 children under five with acute gastroenteritis over one year.
  • Enzyme immunoassay (EIA) was used to detect Rotavirus antigen.
  • Latex agglutination (LA) was used to compare sensitivity with EIA.

Main Results:

  • Rotavirus antigen was detected in 38.7% of the faecal samples.
  • Co-infections with other pathogens were observed in positive samples.
  • Enzyme immunoassay (EIA) demonstrated higher sensitivity compared to latex agglutination (LA).

Conclusions:

  • This study reports the first documented incidence of Rotavirus infection in children in Nepal.
  • Enzyme immunoassay (EIA) is a more sensitive method for Rotavirus antigen detection than latex agglutination (LA).
  • Findings highlight the importance of Rotavirus surveillance and accurate diagnostics in pediatric populations.
Abstract

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