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Updated: Jul 13, 2026

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

CCDC98 targets BRCA1 to DNA damage sites

Zixing Liu1, Jiaxue Wu, Xiaochun Yu

  • 1Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher Place, BSRB 1520, Ann Arbor, Michigan 48109, USA.

Nature Structural & Molecular Biology
|July 24, 2007
PubMed

Insights

CCDC98 is a newly identified BRCA1 binding partner. It is crucial for recruiting BRCA1 to DNA damage sites and activating cell cycle checkpoints, thus mediating BRCA1

Area of Science:

  • Molecular biology
  • Cellular biology
  • Cancer research

Background:

  • The protein Breast cancer-1 (BRCA1) plays a vital role in DNA damage response.
  • The precise mechanism of BRCA1 recruitment to DNA damage sites is not fully understood.
  • RAP80, a ubiquitin-binding protein, has been identified as essential for BRCA1 translocation following DNA damage.

Purpose of the Study:

  • To identify novel components of the BRCA1-RAP80 complex.
  • To elucidate the role of CCDC98 in BRCA1-mediated DNA damage response.
  • To investigate CCDC98's function in BRCA1 translocation and checkpoint activation.

Main Methods:

  • Co-immunoprecipitation assays to identify protein interactions.
  • Immunofluorescence microscopy to visualize BRCA1 foci formation.
  • Cell cycle analysis to assess G2/M checkpoint activation.

Main Results:

  • CCDC98 was identified as a novel binding partner within the BRCA1-RAP80 complex.
  • CCDC98 directly mediates the association between BRCA1 and RAP80.
  • CCDC98 is essential for the formation of BRCA1 foci at DNA damage sites.
  • CCDC98 controls BRCA1-dependent activation of the G2/M cell cycle checkpoint.

Conclusions:

  • CCDC98 is a critical component that bridges BRCA1 and RAP80.
  • CCDC98 plays a significant role in the DNA damage response pathway by regulating BRCA1 function.
  • CCDC98 is a key mediator of BRCA1's role in maintaining genomic stability.

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