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Related Experiment Video

Updated: Jul 13, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Codon engineering for improved antibody expression in mammalian cells.

Jill M Carton1, Tina Sauerwald, Pam Hawley-Nelson

  • 1Department of Molecular Discovery Technologies, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA. jcarton2@cntus.jnj.com

Protein Expression and Purification
|July 25, 2007
PubMed
Summary

Optimizing codon usage in recombinant antibodies significantly enhances protein expression in mammalian systems. Human codon optimization (HCO) of CNTO 888 improved antibody production cell line development.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Engineering

Background:

  • Mammalian expression systems are crucial for biotherapeutic research and manufacturing.
  • The impact of coding sequence variation on protein expression in mammalian cells is not well understood.
  • Recombinant antibody production often involves sequences optimized for bacterial systems.

Purpose of the Study:

  • To investigate the effect of codon engineering on recombinant antibody expression in mammalian systems.
  • To compare expression levels of native, native codon exchanged (NCE), and human codon optimized (HCO) variants of CNTO 888.
  • To assess the potential for improving antibody production cell line development through codon optimization.

Main Methods:

  • Engineered two coding variants (NCE and HCO) of a human monoclonal antibody (CNTO 888) with bacterial codon bias.
  • Transfected mammalian host cells with the parental and engineered antibody sequences.
  • Measured antibody expression levels in stable cell lines.

Main Results:

  • The human codon optimized (HCO) variant of CNTO 888 demonstrated the highest expression levels.
  • HCO variant yielded the highest expressing cell lines and average expression across screened populations.
  • Codon engineering positively impacted the development of a CNTO 888 production cell line.

Conclusions:

  • Codon engineering, specifically human codon optimization, can significantly enhance recombinant antibody expression in mammalian systems.
  • This approach offers a valuable strategy for improving the efficiency of biotherapeutic protein production.
  • Optimizing coding sequences is a key factor in developing robust mammalian cell lines for antibody manufacturing.