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Host range temperature-sensitive mutants of herpes simplex virus type 2.

D Westmoreland, F Rapp

    Journal of Virology
    |April 1, 1976
    PubMed
    Summary

    Two herpes simplex virus type 2 (HSV-2) mutants were analyzed. Mutant 69 produced noninfectious particles that interfered with wild-type HSV-2 replication, while mutant 74 showed delayed DNA synthesis and increased UV sensitivity.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Herpes simplex virus type 2 (HSV-2) is a significant human pathogen.
    • Temperature-sensitive mutants are valuable tools for studying viral replication and function.
    • Cell-dependent temperature sensitivity in HSV-2 mutants offers insights into host-virus interactions.

    Purpose of the Study:

    • To characterize the biological properties of two HSV-2 small-plaque mutants.
    • To compare the behavior of these mutants with the parental wild-type virus in various assays.
    • To investigate the impact of specific mutations on viral replication, particle formation, and UV sensitivity.

    Main Methods:

    • Comparative biological assays of parental and mutant HSV-2 strains.
    • Analysis of viral particle production and infectivity under permissive and nonpermissive conditions.
    • Assessment of viral DNA synthesis and progeny production kinetics.
    • Evaluation of sensitivity to UV irradiation and viral transformation efficiency.

    Main Results:

    • Mutant 69 produced abundant, noninfectious HSV-2 particles that interfered with wild-type replication.
    • Mutant 74 exhibited delayed viral DNA synthesis and progeny production, even at permissive temperatures.
    • Both mutants displayed increased sensitivity to UV irradiation compared to the parental virus, with mutant 74 being particularly sensitive.
    • Mutant 74 demonstrated high transforming efficiency at lower UV doses than wild-type HSV-2.

    Conclusions:

    • Mutant 69's noninfectious particles can act as interfering agents, suggesting roles in viral pathogenesis or therapeutic strategies.
    • Mutant 74's defects in DNA synthesis and enhanced UV sensitivity highlight its potential for studying viral genome replication and DNA repair mechanisms.
    • These HSV-2 mutants provide distinct models for dissecting viral gene function and host-pathogen interactions.

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