Related Experiment Video

Updated: Jul 13, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Some Microbiological Aspects of Staphylococcal Mastitis

N Joshi, D G Dale

    Canadian Journal of Comparative Medicine and Veterinary Science
    |March 1, 1963
    PubMed

    Insights

    Detecting coagulase-positive staphylococci in milk is crucial. Mannitol fermentation under anaerobic conditions and urease production are reliable indicators, unlike gelatin liquefaction or Tellurite Glycine agar.

    Area of Science:

    • Microbiology
    • Food Safety
    • Dairy Science

    Background:

    • Coagulase-positive staphylococci are significant contaminants in milk.
    • Accurate detection methods are essential for ensuring milk safety and quality.

    Purpose of the Study:

    • To evaluate the reliability of various methods for detecting coagulase-positive staphylococci in milk.
    • To identify optimal diagnostic techniques for staphylococcal contamination in dairy products.

    Main Methods:

    • Assessment of mannitol fermentation under aerobic and anaerobic conditions.
    • Evaluation of hemolytic activity on bovine and sheep blood agar.
    • Testing of gelatin liquefaction and urease production.
    • Analysis of Tellurite Glycine agar medium performance.

    Main Results:

    • Anaerobic mannitol fermentation improves reliability for detecting coagulase-positive staphylococci.
    • Beta-hemolytic staphylococci are predominant among hemolytic strains, with enhanced expression on sheep blood agar.
    • Urease production is characteristic of most coagulase-positive staphylococci.
    • Gelatin liquefaction and Tellurite Glycine agar are unreliable for selection.

    Conclusions:

    • Optimized mannitol fermentation (anaerobic) and urease production are recommended for identifying coagulase-positive staphylococci in milk.
    • Gelatin liquefaction and Tellurite Glycine agar are unsuitable for this purpose.
    • Understanding hemolytic patterns aids in staphylococcal strain characterization.

    More Related Videos

    Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)12:18

    Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)

    Published on: February 9, 2011

    Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic08:51

    Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

    Published on: September 4, 2012

    Related Concept Videos

    Clinical Significance of Antibiotic Resistance01:25

    Clinical Significance of Antibiotic Resistance

    Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
    Staphylococcal Skin Infections01:29

    Staphylococcal Skin Infections

    Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
    Mechanism of Antibiotic Resistance in MRSA01:25

    Mechanism of Antibiotic Resistance in MRSA

    Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
    The Skin Microbiota01:27

    The Skin Microbiota

    The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
    Surface Membrane Barriers01:18

    Surface Membrane Barriers

    The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
    The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
    Special Staining Techniques01:13

    Special Staining Techniques

    Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...