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Updated: Jul 13, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Differential expression of wheat genes during cold acclimation.

N K Christov¹, S Yoneyama, Y Shimamoto

¹Crop Cold Tolerance Research Team, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-08555, Japan.

Tsitologiia I Genetika

|July 26, 2007

Summary

Researchers identified three novel genes, high mobility globular protein (HMGB1), glycine-rich RNA-binding protein (TaGRP2), and dehydrin (DHN14), involved in winter wheat

Related Experiment Videos

Last Updated: Jul 13, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Area of Science:

Plant molecular biology
Crop science
Gene expression analysis

Background:

Winter wheat (Triticum aestivum) enhances freezing tolerance via cold acclimation (CA).
Understanding CA-associated genes is crucial for improving crop resilience.
Novel gene identification aids in elucidating molecular mechanisms of plant adaptation.

Purpose of the Study:

To identify and characterize novel genes associated with cold acclimation in winter wheat.
To investigate the differential regulation of these genes under cold stress, drought, and abscisic acid (ABA) treatment.

Main Methods:

Differential screening of a winter wheat crown tissue cDNA library from cold-acclimated plants.
Nucleotide sequence analysis to identify gene products.
Quantitative analysis of mRNA accumulation under various stress conditions (cold, drought, ABA).

Main Results:

Three novel CA-associated cDNAs were identified, encoding high mobility globular protein 1 (HMGB1), glycine-rich RNA-binding protein 2 (TaGRP2), and LEA D-11 dehydrin 14 (DHN14).
mRNA accumulation of these genes was differentially regulated during 14 days of CA.
DHN14 mRNA rapidly accumulated in response to drought and ABA, while HMGB1 and TaGRP2 mRNA levels remained unchanged.

Conclusions:

HMGB1, TaGRP2, and DHN14 are novel genes involved in winter wheat's cold acclimation response.
DHN14 exhibits distinct regulatory patterns, suggesting a specific role in drought and ABA signaling during cold stress.
Further research into the functions of these genes can inform strategies for enhancing crop cold tolerance.