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[Establishment of a selective inactivation adenosine A2A receptors mice model]
Jian-Hong An1, Wei Li, Song Xie
1Institute of Surgery and Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
Objective:
To establish a mice model with selective inactivation adenosine A2A receptors (A2ARs) in peripheral white blood cells (PWBC).
Methods:
A2ARs were selectively inactivated in PWBCs by transplanting bone marrow cells (BMCs) from A2AR knockout (KO) mice into their wild type (WT) littermates after a single total body irradiation of 9.5 Gy or fractionated total body irradiation of 6.2 Gy x 2. The efficiency of reconstitution of bone marrow-derived cells in chimeric mice was assessed.
Results:
PCR band patterns changed from the recipient pattern (one band of 330 bp) to the donor (two bands of 300 and 330 bp) pattern. Immunohistochemistry analysis showed that 10.21% of cells were A2AR+ in PWBCs in KO--> WT mice, whereas 96.72% of cells were A2AR+ in WT mice. The survival rates of mice irradiated with 6.2 Gy x 2 and transplanted with more than 6 x 10(6) BMCs were about 91%.
Conclusion:
A murine model of selective inactivation adenosine A2A receptors in PWBCs was established successfully.
Insights
A new mouse model successfully inactivates adenosine A2A receptors (A2ARs) in peripheral white blood cells (PWBCs). This model is crucial for studying A2AR function in immune cells and related diseases.
Area of Science:
- Immunology
- Pharmacology
- Genetics
Context:
- Adenosine A2A receptors (A2ARs) play a significant role in immune cell function.
- Selective manipulation of A2ARs in specific cell types is essential for understanding their physiological roles.
- Current models may lack specificity in targeting A2ARs within the peripheral white blood cell population.
Purpose:
- To develop a novel murine model for the selective inactivation of adenosine A2A receptors (A2ARs) in peripheral white blood cells (PWBCs).
- To establish a reliable method for creating chimeric mice with targeted A2AR deficiency in hematopoietic-derived cells.
Summary:
- A2ARs were selectively inactivated in PWBCs using a bone marrow transplantation (BMT) strategy.
- Mice received bone marrow cells (BMCs) from A2AR knockout (KO) donors into irradiated wild-type (WT) recipients.
- Successful reconstitution and selective A2AR inactivation in PWBCs were confirmed via PCR and immunohistochemistry, with high survival rates observed.
Impact:
- This model provides a powerful tool for investigating the specific functions of A2ARs in PWBCs.
- It will facilitate research into the role of A2ARs in immune responses, inflammation, and related pathologies.
- The established model enables targeted studies on A2AR-mediated signaling pathways within the immune system.
