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Updated: Jul 13, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Heterogeneous expression of tumor-associated genes in disseminated breast cancer cells
Veit Zieglschmid1, Christiane Hollmann, Bertha Gutierrez
1AdnaGen AG, Ostpassage 7, D-30853 Langenhagen, Germany.
Background:
Gene expression profiles were determined to demonstrate heterogeneity of viable disseminated tumor cells (DTC) in the blood of breast cancer patients.
Patients And Methods:
All patients (n = 48) suffered from metastatic disease (M1) and were treated with chemotherapy and/or Herceptin, respectively. Blood samples were analyzed by a DTC detection assay consisting of immunomagnetic tumor cell selection combined with expression profiling of the tumor-associated transcripts GA733-2, MUC-1, HER-2 and Claudin-7. In addition, the correlation of HER-2 expression in DTC with histopathologically determined HER-2 status in distant metastases and primary tumors in selected cases was investigated.
Results:
DTC were detected in 69% (p < 0.0001) of breast cancer patients. The expression profiles were shown to be heterogeneous within different patients and even within the follow-up period of patients, reflecting the expected heterogeneity of DTC. Furthermore, preliminary results showed a correlation between HER-2 gene expression in DTC and HER-2 overexpression in tumor tissue of distant metastases.
Conclusion:
The results suggest a clinical value of the DTC detection assay with respect to a more precise characterization of individual cancer disease and selection for therapy. More emphasis should be placed on HER-2 expression in DTC as a possible precursor of distant metastases.
