[Effects of lanthanum on inhibition of lipopolysaccharide induced NF-kappaB activation]

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A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
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A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

Fei Guo1, Yang Wang, Guo-Hui Li

  • 1Burn Institute of Nanchang University, Nanchang 330006, P. R. China.

Zhonghua Shao Shang Za Zhi = Zhonghua Shaoshang Zazhi = Chinese Journal of Burns
|July 26, 2007
PubMed
Abstract

Insights

Lanthanum (La3+) inhibits lipopolysaccharide (LPS) induced activation of NF-KB in mouse macrophages. This suggests lanthanum may be a potential therapeutic agent for inflammatory conditions driven by NF-KB signaling.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Lipopolysaccharide (LPS) triggers inflammatory responses by activating the NF-KB signaling pathway.
  • NF-KB activation in macrophages leads to the production of pro-inflammatory cytokines like TNF-alpha.
  • Understanding modulators of NF-KB activation is crucial for developing anti-inflammatory strategies.

Purpose of the Study:

  • To investigate the effect of lanthanum (La3+) on LPS-induced NF-KB activation in murine peritoneal macrophages.
  • To determine if lanthanum can inhibit the inflammatory cascade initiated by LPS.

Main Methods:

  • Murine peritoneal macrophages were cultured and treated with LPS, La3+, or combinations thereof.
  • NF-KB/p65 translocation to the nucleus was assessed using immunofluorescence.
  • NF-KB/p65 DNA binding activity and nuclear expression were measured via TransAM assay and Western blotting.
  • Cytoplasmic IkappaBalpha levels and supernatant TNF-alpha concentrations were quantified using Western blotting and ELISA, respectively.

Main Results:

  • LPS induced significant nuclear translocation and increased expression/activity of NF-KB/p65, along with decreased cytoplasmic IkappaBalpha.
  • Lanthanum treatment, alone or in combination with LPS, inhibited NF-KB/p65 nuclear translocation and activation.
  • Lanthanum significantly reduced LPS-induced TNF-alpha secretion by macrophages.

Conclusions:

  • LPS activates NF-KB signaling in macrophages, leading to increased TNF-alpha production.
  • Lanthanum effectively inhibits LPS-induced NF-KB activation and subsequent inflammatory cytokine release.
  • Lanthanum demonstrates potential as an inhibitor of inflammatory pathways mediated by NF-KB.

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