Related Experiment Video
Updated: Jul 13, 2026
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
Demonstration of changes in plasma cell subsets in multiple myeloma
Michael John Ayliffe1, Faith Elizabeth Davies, David de Castro
1Department of Immunology, St Helier Hospital Wrythe Lane, Carshalton, Surrey, UK. michael.ayliffe@onetel.net
Abstract:
Increases in free light chain (FLC) production are associated with disease progression in multiple myeloma (MM). Using a double immunofluorescence staining method to produce a differential count of plasma cells in bone marrow, single populations were demonstrated, containing intact monoclonal immunoglobulins (M-Igs) in 74% and FLCs only in 8% of cases. However, 18% contained a mixture of both cell populations. Progression from cells making intact M-Ig to cells restricted to FLC only production occurred in individual cases during the course of their disease. The presence of FLC only cells was associated with shortened survival.
