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Published on: April 22, 2019
[Effects of garlicin on NIH3T3 cell proliferation and collagen synthesis]
Hai-Xiao Zhang1, Zai-Xiang Shi, Hai-Zhong Jia
1National Integrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital, Ministry of Health, Beijing.
Objective:
To investigate the effects of garlicin on fibroblasts proliferation and type I collagen synthesis and explore its anti-fibrosis mechanism.
Methods:
Garlicin was added into the culture fluid of NIH3T3 cell, taking Radix Salviae miltiorrhizae as the control medicine. The spiking of H3-thymidine DNA was detected, also the hydroxyproline (HOP) concentration in the culture fluid by alkali digestion method and the protein expression of type I collagen in NIH3T3 cells by immunofluorescent staining.
Results:
The NIH3T3 cell growth and proliferation rate were obviously reduced after garlicin treatment concentration-dependently in range of 0.2 - 5 microg/mL; HOP level and protein expression of type I collagen also lowered.
Conclusion:
Garlicin could inhibit NIH3T3 cell proliferation, reduce the synthesis and protein expression of type I collagen so as to exert the anti-fibrosis effect.
