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Updated: Jul 13, 2026

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
[Pollutant components and microbial community structure of oil-polluted soils after converted from paddy field to
Rui-xi Ren1, Ying Zhang, Hui Li
1Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. renruixia4@hotmail.com
Abstract:
With the oil-polluted soils after converted from paddy field to upland field for different years in the Shenfu wastewater irrigation area of Shenyang as test materials, the concentrations of their oil pollutants were determined, and the microbial community structure was investigated by denaturing gradient gel electrophoresis (DGGE) and phospholipids fatty acid (PLFA) methods. The results showed that the proportions of total polynuclear aromatic hydrocarbons (PAHs) in total petroleum hydrocarbons (TPHs) and of high molecular weight PAHs in total PAHs increased with the increasing years of field conversion. Total phospholipid fatty acids (PLFAs) had a significant positive correlation with total TPHs, but no correlation with total PAHs. Both DGGE and PLFA analyses indicated that the community structure of soil microbes was mainly correlated with geographical location, and changed obviously when the concentrations of oil pollutants in soil increased to a definite level.
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