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Published on: July 27, 2021
SNP discovery, expression and association analysis for the SDHD gene in pigs
S E F Guimaraes1, M F Rothschild, D Ciobanu
1Department of Animal Science and Center for Integrated Animal Genomics, Iowa State University, Ames, IA, USA. sfacioni@ufv.br
Summary
Researchers investigated the SDHD gene in pigs, identifying single nucleotide polymorphisms (SNPs) and analyzing gene expression. Both SDHD SNPs and expression levels were linked to important traits like growth and meat quality in pigs.
Area of Science:
- Animal Genetics
- Molecular Biology
- Meat Science
Background:
- The Succinate Dehydrogenase D (SDHD) gene plays a role in cellular respiration.
- Understanding genetic variations and expression patterns of SDHD can provide insights into livestock productivity.
- Commercial pig breeds exhibit diverse growth potentials, making them suitable models for genetic studies.
Purpose of the Study:
- To identify single nucleotide polymorphisms (SNPs) in the SDHD gene.
- To analyze the expression levels of SDHD in relation to growth and meat quality traits.
- To investigate the association of SDHD genetic variations and expression with economically important traits in pigs.
Main Methods:
- Genomic DNA extraction and sequencing to identify SNPs in the SDHD gene.
- Quantitative real-time PCR (qPCR) to measure normalized SDHD gene expression.
- Statistical analysis to correlate SNPs and expression with growth, carcass composition, meat quality, and sensory traits.
Main Results:
- Three SNPs were identified in the SDHD gene, including two novel SNPs in the 3'-UTR region.
- Normalized SDHD gene expression showed significant correlations with growth, meat quality, and sensory traits (p<0.05).
- Both identified SDHD SNPs and gene expression levels were significantly associated with growth, carcass composition, meat quality, and sensory traits (p<0.05) in commercial pigs and a resource population.
Conclusions:
- The SDHD gene harbors genetic variations (SNPs) and its expression is linked to key production and quality traits in pigs.
- SDHD SNPs and expression are valuable genetic markers for improving growth performance and meat quality in swine breeding programs.
- Further research can explore the functional mechanisms underlying the association between SDHD and pig traits.
Related Concept Videos
01:11Genome-wide Association Studies-GWAS
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
01:05Single Nucleotide Polymorphisms-SNPs
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...