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Related Experiment Videos

Human dopamine D5 receptor pseudogenes.

T Nguyen1, J Bard, H Jin

  • 1Department of Pharmacology, University of Toronto, Ontario, Canada.

Gene
|December 30, 1991
PubMed
Summary
This summary is machine-generated.

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Researchers identified two new intronless genes in the human genome that are similar to the DRD5 receptor gene. These genes, PG-1 and PG-2, are DRD5 pseudogenes and cannot produce functional dopamine receptors.

Area of Science:

  • Genomics
  • Molecular Biology
  • Neuroscience

Background:

  • Five human dopamine receptor genes (DRD1-DRD5) have been identified.
  • DRD1 and DRD5 are encoded by intronless genes.

Purpose of the Study:

  • To identify other intronless genes with sequence similarity to the DRD5 gene.
  • To characterize these novel genes and their potential function.

Main Methods:

  • Polymerase chain reaction (PCR) amplification of human genomic DNA using DRD5-specific oligonucleotides.
  • Screening of human genomic libraries to isolate full-length clones.
  • Sequence analysis and comparison of deduced amino acid sequences.

Main Results:

  • Two independent intronless genes, PG-1 and PG-2, sharing identity with DRD5 were isolated.

Related Experiment Videos

  • PG-1 and PG-2 share 91% and 92% amino acid identity with DRD5, respectively.
  • Sequence differences in coding regions indicate PG-1 and PG-2 are non-functional pseudogenes.

    • Conclusions:

    • The human genome contains at least two DRD5 pseudogenes.
    • These pseudogenes are distinct intronless genes with high sequence similarity to DRD5.
    • In situ chromosomal hybridization confirmed the presence of two pseudogenes.