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Related Experiment Videos

Bead-based immunoassays using a micro-chip flow cytometer.

David Holmes1, Joseph K She, Peter L Roach

  • 1School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, UK SO17 1BJ. dh2@ecs.soton.ac.uk

Lab on a Chip
|July 27, 2007
PubMed
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A novel microfluidic flow cytometer analyzes protein-bound beads for antibody detection. This microfabricated device offers comparable performance to commercial systems for quantitative immunoassays.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Analytical Chemistry

Background:

  • Traditional flow cytometry is essential for cell and particle analysis.
  • Microfabrication enables miniaturization of analytical devices for enhanced portability and reduced cost.
  • Quantitative immunoassays are crucial for diagnostics and research.

Purpose of the Study:

  • To develop and validate a microfabricated flow cytometer for analyzing immobilized protein-bound polymer beads.
  • To quantitatively assay antibody binding to surface-immobilized antigens using the developed microfluidic device.
  • To compare the performance of the microfabricated system with a commercial flow cytometer.

Main Methods:

  • Fabrication of a microfluidic chip for bead analysis.
  • Utilized negative dielectrophoresis for particle focusing within the detection zone.

Related Experiment Videos

  • Employed impedance measurements for particle sizing and fluorescence signal triggering.
  • Measured antibody binding via fluorescence at 532 nm and 633 nm, with implemented spectral compensation.

    • Main Results:

    • Successfully analyzed micron-sized polymer beads with immobilized fluorescently labeled proteins.
    • Quantitatively assayed antibody binding to surface-immobilized antigens.
    • Demonstrated comparable data output to a commercial flow cytometer (BD-FACSAria).

    Conclusions:

    • The developed microfabricated flow cytometer is a viable platform for quantitative immunoassays.
    • The device integrates particle manipulation, sizing, and fluorescence detection on a microfluidic chip.
    • This technology shows promise for portable and cost-effective biological analyses.