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Updated: Jul 13, 2026
09:02
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
Angiogenesis factor pattern differs in acute lymphoblastic leukemia and chronic lymphocytic leukemia
Salah Aref1, O Salama, S Shamaa
1Hematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura, Egypt. salaharef@yahoo.com
Hematology (Amsterdam, Netherlands)
|July 27, 2007
Summary
Angiogenesis factors like VEGF and MMP-9 are lower in B-lineage acute lymphoblastic leukemia (B-ALL) but higher in B-chronic lymphocytic leukemia (B-CLL). Their levels change with remission, suggesting different roles in these leukemias.
Area of Science:
- Oncology
- Hematology
- Molecular Biology
Background:
- Angiogenesis is crucial for solid tumor growth and progression.
- The role of angiogenic cytokines in lymphoid leukemia remains unclear.
- Investigating angiogenic profiles in B-cell leukemias is essential for understanding disease mechanisms.
Purpose of the Study:
- To investigate the systemic levels of key angiogenic regulators in B-lineage acute lymphoblastic leukemia (B-ALL) and B-chronic lymphocytic leukemia (B-CLL).
- To compare these levels between patients and healthy controls, and assess changes during remission.
- To explore correlations between angiogenic factors and leukemia cell counts.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure serum levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-alpha), endostatin, and matrix metalloproteinase-9 (MMP-9).
- Measurements were taken at diagnosis and during remission in patients with B-ALL and B-CLL.
- Statistical analyses were performed to compare patient groups with controls and to assess correlations with clinical parameters.
Main Results:
- In B-ALL, serum VEGF and MMP-9 were significantly lower at diagnosis and increased towards control levels in remission.
- In B-CLL, serum VEGF, MMP-9, and TNF-alpha were significantly higher at diagnosis and decreased towards control levels in remission.
- Endostatin levels did not significantly differ from controls in either leukemia type.
- Correlations with platelet counts were observed in B-ALL, while B-CLL showed correlations with white blood cell and bone marrow lymphocyte counts.
Conclusions:
- The regulation of angiogenic factors (VEGF, TNF-alpha, MMP-9) differs significantly between adult B-ALL and B-CLL.
- These findings highlight distinct angiogenic mechanisms driving these two B-cell malignancies.
- Further research into these differences may reveal novel therapeutic targets.
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