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Differential gene expression by styrene in rat reproductive tissue
Jee Hye Han1, Chang-Su Choi, Mie Young Kim
1College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
Journal of Toxicology and Environmental Health. Part A
|July 27, 2007
Summary
Styrene exposure in male rats suppressed clusterin gene expression, potentially causing reproductive toxicity. This study identified key gene changes in the testis following styrene treatment.
Area of Science:
- Toxicology
- Molecular Biology
- Reproductive Science
Background:
- Styrene is a widely used industrial chemical.
- High styrene doses can cause male reproductive toxicity, including infertility and sperm abnormalities.
Purpose of the Study:
- To elucidate the molecular mechanisms of styrene-induced male reproductive toxicity.
- To identify genes differentially expressed in rat testes following styrene exposure.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) with annealing control primers (ACPs).
- Isolation and treatment of male rat testes with styrene.
- Cloning and sequencing of differentially expressed genes.
Main Results:
- Six genes showed differential expression in styrene-treated rats.
- Five genes were induced, including testis-specific expressed gene 101, protein kinase C, H+-ATPase isoform 2, peroxiredoxin 1, and aquaporin 9.
- Clusterin gene expression was significantly suppressed by styrene in a concentration-dependent manner, primarily in the testis.
Conclusions:
- Repression of clusterin gene expression is a key mechanism in styrene-mediated male reproductive toxicity.
- Styrene's effects on gene expression in the testis contribute to its toxicity.
